What does a hematocrit centrifuge do?

What does a hematocrit centrifuge do?

Microhematocrit centrifuges are used for determination of volume fractions of erythrocytes (red blood cells) in blood and for separation of micro volumes of blood and solutions. It provides hematocrit values (HCT) for testing in biochemistry, immunity, genetics, blood separation and for general clinical tests.

What does insufficient centrifugation do to hematocrit?

Artifact: Insufficient centrifugation speed may falsely increase the PCV if the RBC do not pack properly. With false increases in MCV (e.g. storage of blood), the HCT will be falsely increased, but usually the results do not exceed the upper reference limit (however a mildly anemic animal may no longer appear anemic).

What hematocrit would indicate polycythemia?

Hemoglobin levels greater than 16.5 g/dL (grams per deciliter) in women and greater than 18.5 g/dL in men suggest polycythemia. In terms of hematocrit, a value greater than 48 in women and 52 in men is indicative of polycythemia.

What is the centrifugation requirement for Microhematocrit determination?

For the microhematocrit method, after filling the blood in a capillary tube, the two ends of the tube (commonly 75 mm long, 1 mm diameter) are sealed with clay sealant or heat. Then, it is centrifuged at a rate of 11000 to 12000 rpm for 4 to 5 min.

When is a Microhematocrit performed?


  1. Fill two capillary tubes approximately 2/3 to 3/4 full with the well-mixed blood sample.
  2. Seal the dry end of the capillary tube by placing it into the sealing clay at a 90o angle.
  3. Place the capillary tubes in the microhematocrit centrifuge with the sealed end toward the periphery.
  4. Centrifuge for five minutes.

How much is hematocrit centrifuge?

Micro Hematocrit Centrifuge at Rs 75000/unit | Hematocrit Centrifuges | ID: 20671425712.

What is spun hematocrit?

Spun hematocrit is 1% to 3% higher than the hematocrit from automated instrument due to plasma that is trapped in the erythrocytes in the normal situation.

When a capillary tube is centrifuged for a hematocrit reading the blood will separate into three layers from the bottom These layers are?

When a capillary tube is centrifuged for a hematocrit reading, the blood will separate into three layers. From the bottom, these layers are: red blood cells, buffy coat, plasma.

How do you diagnose polycythemia?

Polycythaemia can be diagnosed by carrying out a blood test to check:

  1. the number of red blood cells in your blood (red blood cell count)
  2. the amount of space the red blood cells take up in the blood (haematocrit level)

How do you evaluate polycythemia?

Polycythemia is defined as an elevation in hemoglobin levels (>16.0 g/dL in women or >16.5 g/dL in men; to convert to g/L, multiply by 10.0) or hematocrit (>48% in women or >49% in men).

Why is it important to read the Microhematocrit soon after the centrifuge stops?

Why is it important to read the microhematocrit soon after the centrifuge stops? RBCs being to settle out, causing falsely high results. How does hemolysis affect the microhematocrit? RBC contents lyse out back into plasma, causing falsely low results.

What is the importance of centrifuging a Microhematocrit tube?

When centrifuging a microhematocrit tube, it is important to: Plug one end of the tube with clay. What instrument would the technician use to measure both the urine specific gravity and the protein levels of blood?

What does Microhematocrit measure?

Medical Definition of microhematocrit 1 : a procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin. 2 : a hematocrit value obtained by microhematocrit a microhematocrit of 37 percent.

What is high speed centrifuge?

Definition: A separation method where components of a sample are separated on the basis of their density in a centrifuge according to the centrifugal force they experience. Samples are spun at >5000 rpm.

How does a lab centrifuge work?

A centrifuge is used to separate particles suspended in a liquid according to particle size and density, viscosity of the medium, and rotor speed. Within a solution, gravitational force will cause particles of higher density than the solvent to sink, and those less dense than the solvent to float to the top.

Why is manual hematocrit higher than automated hematocrit?

Where are the white blood cells in the hematocrit tube after the tube is centrifuged?

In the second phase of the procedure, the tube is centrifuged so that its contents separate into three layers—packed red blood cells (erythrocytes) at the bottom, a reddish gray layer of white blood cells (leukocytes) and platelets in the middle, and plasma at the top.

Does high hematocrit mean polycythemia?

Polycythemia is normally reported in terms of increased hematocrit (hematocrit is the ratio of the volume of red blood cells to the total volume of blood) or hemoglobin concentration (hemoglobin is a protein responsible for transporting oxygen in the blood).

What labs are elevated in polycythemia?

If you have polycythemia vera, blood tests might reveal:

  • More red blood cells than normal and, sometimes, an increase in platelets or white blood cells.
  • A greater percentage of red blood cells that make up total blood volume (hematocrit measurement)