What is ExPASy translate tool?
Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.
Which tool is accessed by ExPASy platform?
It provides access to a variety of databases and analytical tools dedicated to proteins and proteomics. ExPASy databases include SWISS-PROT and TrEMBL, SWISS-2DPAGE, PROSITE, ENZYME and the SWISS-MODEL repository.
Why are there 3 possible reading frames?
Genetic code During transcription, the RNA polymerase read the template DNA strand in the 3′→5′ direction, but the mRNA is formed in the 5′ to 3′ direction. The mRNA is single-stranded and therefore only contains three possible reading frames, of which only one is translated.
How does DNA translation work?
Translation is the process that takes the information passed from DNA as messenger RNA and turns it into a series of amino acids bound together with peptide bonds. It is essentially a translation from one code (nucleotide sequence) to another code (amino acid sequence).
What is RNA translation?
Translation is the process by which a protein is synthesized from the information contained in a molecule of messenger RNA (mRNA).
What are 3 steps of translation?
Translation of an mRNA molecule occurs in three stages: initiation, elongation, and termination.
What is blast and how is it used?
BLAST is an acronym for Basic Local Alignment Search Tool and refers to a suite of programs used to generate alignments between a nucleotide or protein sequence, referred to as a “query” and nucleotide or protein sequences within a database, referred to as “subject” sequences.
What is UniProt database?
The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The UniProt databases are the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters (UniRef), and the UniProt Archive (UniParc).
What is NCBI used for?
NCBI is now a leading source for public biomedical databases, software tools for analyzing molecular and genomic data, and research in computational biology. Today NCBI creates and maintains over 40 integrated databases for the medical and scientific communities as well as the general public.
Is NCBI free?
Click on the PubMed Central link or a Publisher’s link to access the full text of the article. Articles in PubMed Central are freely available. Articles on Publisher’s websites are either freely available or can be accessed with a fee. Contact the specific publisher for questions about their site.
What are six frames of translations?
Thus, when trying to translate a given piece of DNA, one must try reading the DNA in each of three possible overlapping reading frames in the forward direction and the complementary strand in the reverse direction. This is known as 6-frame translation and you use a web-based resource to perform a 6-frame translation.
What is the difference between ORF and CDS?
The Coding Sequence (CDS) is the actual region of DNA that is translated to form proteins. While the ORF may contain introns as well, the CDS refers to those nucleotides(concatenated exons) that can be divided into codons which are actually translated into amino acids by the ribosomal translation machinery.